Chitosan was extracted from Aspergillus niger mycelium and characterized by several techniques (FTIR, 1HNMR y 13C-CP-MAS-NMR, TGA, DSC and XRD) with a reaction yield of 5.02%, and its average viscosity molecular weight was 1.52 x 105 g/mol. Deacetylation degree was determined by elemental analysis and 1H-NMR, being around 75%. Chitosan films were obtained by film casting, using glycerol and oleic acid as plasticizers, the chitosan films were characterized by SEM and XRD. Degradability in vitro tests under physiological conditions, mechanical tests, and biocompatibility tests on Rattus norvegicus were run, followed by histological studies of connective tissue samples from the site where the films were implanted finding complete absorption of the material and no damage to nearby tissues.