We describe a new species of Strengeriana Pretzmann, 1971, S. villaensis, from Villahermosa, Tolima Department, on the eastern slope of the Central Andes. The genus is endemic to Colombia and is distributed in the Sierra Nevada de Santa Marta and the Western and Central Andes, at elevations ranging from 700 to 2000 m. With the addition of S. villaensis the total number of species in the genus rises to 16. This new species is distinguished from its congeners primarily by the morphology of the first male gonopod, particularly by the shape of the mesial, cephalic and lateral lobes, and the mesial and cephalic processes. We present a key for the identification of the species of the genus based on the morphology of the first male gonopod and the third maxilliped.