The regeneration of bone is one of the main challenges of modern medicine because many diseases including trauma and tumor can cause bone defects. Tissue engineering (TE) is a promising approach to cure these bone diseases since it allows the reconstruction of tissue by colonization and proliferation of a patient is healthy cells is in an artificial extracellular matrix (scaffolds). The aim of this project was to prepare chitosan/hydroxyapatite CH/HA scaffolds, using various ratios and two different methods: powder hydroxyapatite (commercial) and in situ hydroxyapatite, and then compare their properties. The morphology, chemical composition, and mechanical properties were evaluated by Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, and compression tests. The scaffolds obtained showed an interconnected porous structure. Scaffolds with better applications in tissue engineering are the scaffolds with chitosan and hydroxyapatite in situ protocol, due to their better morphology; they allow the cell growth. These scaffolds meet the requirements of tissue engineering.