Abstract From the ethanolic extract of Lentinus edodes (Shiitake) eight known sterols were isolated and identified: ergosterol, ergosterol peroxide, (22E)-ergosta-5,7,9(11), 22-tetraen-3β-ol, (22E)-ergosta-7,9(11),22-trien-3β-ol, (22E)-ergosta-6,8,22-trien-3β-ol, (22E)-norergosta-5,7,9,22-tetraen-3β-ol, 3β,5α-dihydroxy-(22E)-ergosta-7,22-dien-6-one, (22E)-ergosta-4,6,8(14),22-tetraen-3-one and, for the first time in mushrooms, (22E)-ergosta-6,22-diene-3β,5α,8α-triol. Keywords: Lentinus edodes ShiitakemushroomspolyhydroxysterolsGIAO Acknowledgements The authors would like to thank Dirección Nacional de Investigaciones (DINAIN) and Departamento de Química, Universidad Nacional de Colombia for their financial support.
