During July 7th and 8th of 2008, the First Colombian Consensus Conference for the Immunophenotyping of Hematological Malignancies was carried out in Cali, Colombia, with representatives from Colombia, Argentina, Chile, Perú and México. The consensus included professionals from the laboratory field and clinicians from different institutions that routinely operate clinical flow cytometry with an emphasis on lymphoma and leukemia immunophenotyping.In this consensus medical indications for flow cytometry use in the detection of hematological malignancies, technical considerations for staining and samples preparation protocols, evaluation of diagnostic immunophenotyping panels, data acquisition and interpretation strategies were analyzed, and, finally, how to report the results.