This taxonomic list of the Trichoptera larvae references data from the Zoological Voucher Collection at Universidad del Tolima, Colombia (Colección Zoológica de la Universidad del Tolima – CZUT-Ma). This collection includes 14 families and 35 genera of Trichoptera (out of the 15 families and 45 genera currently registered in Colombia).The most abundant families were Hydropsychidae, Hydroptilidae and Leptoceridae. The most abundant genera were Smicridea, Leptonema and Hydroptila. Regarding altitudinal distribution of the taxa found in the collection, Neotrichia, Leucotrichia and Smicridea exhibited the widest distribution, since they were found from 244m to 3533m. Results from a correspondence analysis performed on the taxa vs Holdridge life zones data, revealed that the highest species richness of the order Trichoptera in Tolima (Colombia) concentrated in the tropical dry forest, wet pre-mountain forest and lower wet mountain forest zones. © 2015. Acad. Colomb. Cienc. Ex. Fis. Nat. All rights reserved.