The entomopathogenic nematode Heterorhabditis sp. SL0708 (Rhabditida: Heterorhabditidae) isolated from soil in Alcalá, Valle del Cauca (Colombia) was characterised ecologically using Galleria mellonella larvae (L) (Pyralidae: Galleriinae) as hosts. The effect of temperature on the viability, infectivity and reproduction, and of moisture on infectivity and storage in liquid were evaluated in infective juveniles (IJs). Significant differences were found in the viability, infectivity and reproduction of the IJs at different temperatures. No nematodes were recovered at 5 °C and 10 °C, and at 35 °C no infectivity was observed. Average daily nematode recovery was best at 25 °C, and survival of the IJs was low in substrates presenting 13% moisture. The optimal storage temperature for Heterorhabditis sp. SL0708 was between 20 °C and 30 °C, keeping its infectivity for up to 8 weeks.