The objective of this study was to evaluate a manual method to produce two kinds of pure-platelet rich plasma (P-PRP-A and P-PRP-B) in rabbits.Both P-PRP were obtained by drawing blood from the jugular vein into tubes containing sodium citrate as anticoagulant and, centrifuged at 259 g for 6 minutes.The number of concentrated platelets in the P-PRP-A was 716.14 X 10 3 /μL and 630 X 10 3 /μL for P-PRP-B.Cell counts were significantly different (P < 0.05) between whole blood and PRP fractions A and B, for RBC counts (10 6 /μL), WBC (10 3 /μL), PLT (10 3 /μL) and PDW (%) but not for MPV (fL).Gender differences were found in P-PRP-A for RBC (10 6 /μL), which was higher in males, and the MPV (fL), which was higher in females.In fraction P-PRP-B a difference was found for MPV (fL), which was higher in females.The PDW (%) was higher in males compared with females.The platelet collection efficiency was 20.76% for P-PRP-A and 18.27% for P-PRP-B.The platelet concentration considering whole blood was 186.92% for P-PRP-A and 164.44% for P-PRP-B.The protocol described in this study represents a simple method to obtain P-PRP in rabbits for experimental purposes and even for therapeutic use.