We present the first measurement of the integrated forward-backward charge asymmetry in top-quark–top-antiquark pair (t¯t) production in proton-antiproton (p¯p) collisions in the lepton+jets final state. Using a b-jet tagging algorithm and kinematic reconstruction assuming t¯t+X production and decay, a sample of 0.9 fb−1 of data, collected by the D0 experiment at the Fermilab Tevatron Collider, is used to measure the asymmetry for different jet multiplicities. The result is also used to set upper limits on t¯t+X production via a Z′ resonance.Received 7 December 2007DOI:https://doi.org/10.1103/PhysRevLett.100.142002©2008 American Physical Society