ABSTRACT Some studies have recently reported high diploid male frequencies in euglossine bees indicating possible population declines. We estimated the genetic variability and frequency of males that are diploid in five Colombian species of euglossine bees using allozyme markers. Expected heterozygosities ranged from 0.10 to 0.23. Diploid males were found in three species with frequencies ranging from 8 to 32 percent. Our results suggest that some species are more susceptible to environmental changes and anthropogenic pressure.