The aim of this study was to examine the impact of different Mixed Liquor Volatile Suspended Solids (MLVSS) concentrations on membrane fouling,in a submerged Membrane Bioreactor (MBR) at short and longterm MBRoperation for waste water treatment. Three laboratory-scale in a submerged MBR system were operated under critical flux, subcritical flux,and an intermittent suction time and backwashing conditions. At short-term MBR operation with mixed liquors of 4,200 and 6,150 mg MLVSSL−1, the hydraulic resistance of membranes followed a same trajectory with averages of 5.0E+12m−1, whereas for 7,940 mg MLVSSL−1, a highresistance of up to 1.7E+13m−1was obtained. The result showed thathigh biomass concentrations decreased to permeability due to a bio-layer formed in the membrane surface and high Extracellular Polymeric Substance (EPS).