Colombia is a tropical country with high diversity and an agricultural economy, yet their soils are characterized by low pHs and poor phosphorus concentrations. Soil supplementation with chemical fertilizers containing soluble phosphorus is a costly and contaminating practice and for this reason, the aim of this study was to isolate actinobacteria that are able to release soluble phosphate from wild plants of the Eastern Cordillera of Colombia and select strains with high phosphorus solubilizing activity. To screen the isolates of actinobacteria, we used two qualitative assays to determine the efficiency of solubilization by measuring the halo of hydrolysis in a Pikovskaya's agar plate (PVK). A second assay was performed on broth with National Botanical Research Institute's phosphate growth (NBRIP) medium containing bromophenol blue (BPB). Finally, the released soluble phosphate by actinobacteria was quantified using insoluble Ca3(PO4)2 or AlPO4 as sole sources of P. Only five of the tested strains were the best solubilizing strains in the two qualitative assays. The strains T1C, T1H, T3A, T3C, P3E, F1A, F2A and V2B solubilized significantly more phosphorus than the other strains, which was shown for the quantitative assay. Strains T1C, T3A, T3C and F1A are candidates for future studies and to evaluate other plant growth promoting activities. Key words: Screening, phosphate solubilizing, actinobacteria, Colombia.