Introduction: Exposure to pollutants such as pesticides and heavy metals has been linked to health problems. Several studies have revealed the presence of these contaminants in Cali; however, there is no information available about the main routes of exposure and risk of these contaminants.Objective: To estimate the risk associated with the intake of cadmium, lead and mercury, and pesticides 2,4-D and diuron through the consumption of water and food in a population in Cali.Materials and methods: Population and environmental data were obtained, and a risk assessment was performed using United States Environmental Protection Agency guidelines.Results: The concentrations of the evaluated pollutants were below permissible levels as established by the Colombian Ministerio de Ambiente, Vivienda y Desarrollo Territorial (3 μg/L-1 of cadmium; 10 μg/L-1 of lead; 1 μg/L-1 of mercury; 1 μg/L-1 of 2,4 D; 1 μg/L-1 of diuron). Salema butterfish (Peprilus snyderi) samples contained levels of cadmium between 20 and 80 μg/kg-1, which are below the permissible limit set by the World Health Organization (100 μg/kg-1). The results of the risk assessment indicated that the carcinogenic and non-carcinogenic attributable risk to population health from the intake of food contaminants was below the maximum level permitted by the United States Environmental Protection Agency.Conclusions: It is believed that the findings in previous studies on pollutants may have been due to specific contamination events; therefore, monitoring and early warning about water intake is recommended. Furthermore, the report of cadmium being found in fish consumed as food suggests the need for quality control by regulators.