Two-Factor Model of Emotionof Watson and Tellengen has been investigated for the differentiation of anxiety and depression in Europe and North America, but no studies have been reported in middle-aged Cuban population. The objective of this research was to investigate the negative (NA) and positive affectivity (PA) in individuals without psychopathology, predominantly anxious, depressive or mixed symptoms. The sample was 219 subjects; 19.2% with symptoms of anxiety, 17.8% has depression, 23.7% mixed anxiety and depression symptoms, and the rest without symptoms. Positive and negative affect schedule (PANAS) was used to study emotion; and Anxiety and Depression Scale Goldberg (GADS) to differentiate diagnostic subgroups. The one-way ANOVA was used with the method of Tukey Honest Significant Difference. We found that AP was lower in depressive as predicted by the model, but the prediction that the AP would differentiate anxious people without symptoms was fulfilled, which highlights the need to evaluate the models developed in other cultures.