Oceanic islands have special ecosystem dynamics due to their isolation and weather conditions influenced by the sea. This is also the case for Malpelo Island, located at the Colombian Pacific, where previous studies showed an invertebrate composition strongly dominated by predatory species. With the aim of contributing to the knowledge of these organisms on the island, 22 sample plots were surveyed, covering three types of microhabitat, classified according to the type of soil, shadow, and vegetation. Sampling took place between May and June 2006, by direct catches. A total of 1294 specimens from seven classes and 25 orders were obtained. Diversity was determined by microhabitat structure complexity, being more diverse the one that presented the greatest degree of heterogeneity. The order Araneae presented the highest number of species, Coleoptera the highest abundance and Staphylinidae the highest relative abundance. Predators showed the highest richness (45.3 %) and abundance (40.6 %) among all trophic guilds. These findings lent support to the idea that the terrestrial system of Malpelo depends on the sea.