Although calcium titanate has been proposed as a coating for biomedical applications, a characterization of tribological properties simulating human conditions has not been reported. In this work we studied friction and wear mechanism of calcium titanate coating growth onto AISI 304 steel (750 nm thickness) deposited by r.f. magnetron sputtering. It was found that the wear mechanisms of the system is bone adhesion to the coating without detachment of the coating, both dry and in Hank’s solution, with a friction coefficient of 0.84 ± 0.13 and 0.65 ± 0.13, respectively. The wear of the bone was more severe when using a simulated body fluid at 37°C in the pin on disk test.