The mechanisms to obtain and store skin toxins in frogs in of the family Dendrobatidae are not completely understood.In order to contribute to understand how toxins are stored, we provide a histological description of the cutaneous glands of the species Phyllobates bicolor.The skin of two adult frogs was examined through three histological staining techniques (hematoxilin-eosin, PAS and Masson Trichrome) using conventional optic microscopy.The skin of Phyllobates bicolor contains two types of exocrine glands: mucous and serous, which empty their products to the epidermal surface through an intra-epithelial duct that leads to a stoma.The mucous and serous glands and the intercalated ducts are surrounded by a discontinuous sheath of myoepithelial cells, which colapse the lumen of the acinus and the lumen of ducts and facilitate the secretion and release of their content.The serous glands have a polarized syncytium of tall cuboidal or columnar epithelial cells.Both glands have a mixed secretion, thus, the contents of mucous glands tend to be neutral and basophilic, while the contents of the serous glands are basophilic and acidophilic.