Different methods have been studied to mitigate xenograft calcification based on fixation with glutaraldehyde (GLU) or photooxidation (PO). The purpose of this work was to study the effect of PO and GLU concentration and temperature on graft mechanical properties and calcification. Fresh bovine pericardium was treated as follows: G1: untreated; G2: fixed in 0.625% GLU, 4°C, for 30 days; G3: 1.0% GLU, 4°C, 30 days; G4: 3.0% GLU, 4°C, 30 days; G5: 1.0% GLU, 4°C 20 days and 50°C 20 days; G6: 3.0% GLU, 4°C 20 days and 50°C 20 days; G7: PO in metilene blue at 0°C for 8 h. Samples were subjected to tensile strength tests (n=73) or subcutaneously implanted in mice for 40 days (n=83). Calcification of implants was assessed by absorption spectrophotometry (n=55) or von Kossa stain (n=28). ANOVA and Tukey's test were used for statistical analysis. Young modulus (E) and ultimate stress (σ) were significantly different between groups. E was higher in G1, followed by groups 3, 5, 7, 4, 2 and 6. σ was statistically higher in G1 than in all other groups: 5, 4, 7, 6, 2 and 3. There were no significant differences in calcification by spectroscopical analysis. Fixation should preserve properties of fresh tissue. Mechanical properties of fixed xenografts appear to be better at 1.0% GLU than at 0.625% GLU and 3.0% GLU. At 1.0% GLU, fixation at 50°C seems to improve these properties. Subcutaneous calcification was not alleviated by any treatment. As used in this study, PO did not improve mechanical or anticalcification properties.