The critically endangered harlequin frog Atelopus eusebianus is known from a few locations in the Cauca Department, on the western slope of the Central Andes. We report a new locality in Valle del Cauca department, this location is found on the western slope of the Central Andes, located about 63 km north away from the localities known, it´s an important finding due to the need for known the real range of this species.