Constructed wetlands are a very efficient, clean and economical way to remove organic contaminants from waste water. In the whole water cleaning process, some other complex processes, such as physical sedimentation, filtration, chemical precipitation, and material absorption by vegetation, are involved. The Nitrogen absorption efficiency by heliconnia psitacorumm, was studied at laboratory scale in a small reactor simulating a subsurface flow constructed wetland. Chlorophyll increasing was measured by fluorescence, using blue LED, 460 [nm] as excitation light source. Besides, spectral differences were observed in the spectral signal and in its derivative, indicating changes in the plant physiological response.