The objective of this study was to search the presence of antibodies of canine brucellosis in the population of two hostels in the municipality of Envigado (Antioquia, Colombia). For this were chosen 54 dogs of two canine shelters. They were taken blood samples in 10 males and 44 females, and they were processed under the immunochromatography technique for Brucella canis. There was no statistical difference between the parameters hostels in sex and race of each study group and no serologic evidence was found of the presence of canine brucellosis in canines sampled group. The results indicate that although