We provide the first record of the Visored bat Sphaeronycteris toxophyllum for the department of Boyacá, Colombia, in the Eastern Cordillera. This record is based on a female collected in secondary forest at an altitude of 1,100 m. External and skull measurements obtained for this specimen generally agree with those reported in the literature for other Colombian specimens. With the present record, the poorly known bat fauna of Boyacá can be recognized as including at least 34 species.