Ganoderma lucidum, Grifola frondosa and Agaricus blazei mushrooms were cultured in a 14 L bioreactor using low-cost, non-conventional culture media.To produce useful raw material for the development of functional products, the biomass produced in the bioreactors was subjected to the following drying processes: convection, infrared radiation, spray drying and freeze-drying.The highest antioxidant activity (ORAC values) and HMGCoA reductase inhibitory capacity were obtained with G. lucidum, (ORAC value of 10,594±128.1 and HMGCoA reductase inhibitory capacity of 78.22±0.01),followed by G. frondosa (ORAC value of 6,919±231.6and HMGCoA reductase inhibitory capacity of 71.45±1.48)when the biomass was processed via freeze-drying.