SummarySeven virus strains, all isolated from forest mosquitoes collected in eastern Trinidad between 1955 and 1959, have been shown to represent 3 unrelated new viruses, designated Bushbush, Ieri and Lukuni. Neutralizing antibody to Lukuni virus has been demonstrated in human serum. No relationship has been found between either Bushbush or Ieri virus and recognized arboviruses, but Lukuni virus has been found to be related to Anopheles A virus in complement-fixation and neutralization tests. Some of the biological properties of the 3 viruses are described.The technical assistance of Fitzroy James, Shaima Ali, Harold Drysdale, Raymond Manuel, Desmond Seupersad and Enrique Preddie is gratefully acknowledged.