The endemic dendrobatid, Colostethus ruthveni is first reported in the rivers Buritaca, Frío and Piedras from northwestern side of the Colombian Sierra Nevada de Santa Marta and Nabusimake Village in the southeastern side of this massif. The present new reports allow extend the geographical distribution of C. ruthveni to other areas of the Sierra Nevada de Santa Marta where it had not been reported. Our finding suggests that current geographical distribution of this anuran is underestimated. Populations of this anuran are considered endangered (IUCN 2013).