We report new records of the western rounded ear bat Lophostoma occidentalis for the Colombian territory, represented by ten specimens collected on the western portion of the country, in the departments of Cauca, Chocó, and Valle del Cauca, all in the Colombian Pacific Region. The new records of L. occidentalis represent a substantial geographic and ecological extension in the known distribution of this species and corroborate its occurrence in Colombia. In addition, we discuss on the morphological variation of representatives of large Lophostoma species in Colombia.