<i>Background: </i>Protein synthesis is inhibited by oxidative stress. Among the possible causes of this inhibition are the modifications of elongation factor 2 (eEF-2), the protein that catalyzes the translocation of the ribosome through mRNA. eEF-2 is extremely sensitive to oxidative stress caused mainly by lipid peroxidant compounds such as cumene hydroperoxide (CH). <i>Objective:</i> The purpose of this study was to determine whether the antioxidant Trolox prevents the effect of CH on the levels of hepatic eEF-2. <i>Methods:</i> The effect was determined in liver homogenates treated with both compounds. Lipid peroxides and carbonyl content were also measured. <i>Results:</i> The results show that Trolox at certain doses prevents the decrease in the level of eEF-2 caused by CH. <i>Conclusion: </i>Under oxidative stress circumstances, vitamin E can prevent the effect of oxidations on relevant biological processes such as protein synthesis.