Introduction: Cryptosporidium spp. (C. spp) is a protozoan is the fifth cause for diarrhea in Colombia. Objective: To determine the prevalence of C. spp in feces of healthy children under 10 years old by ELISA test. Materials and Methods: Transversal section descriptive observational study was carried out from Centro de Salud Lourdes in Cali, Colombia. Data like identification, age, gender, signs, symptoms, anthropometric measurements, as well as hygienic conditions (drinking water consumption, disposal of feces and home pets presence) were taken from their clinical record. The identification of the C. spp in feces was carried out through ELISA. For this analysis, statistic test were carried out, based on the X2, and due to the low expected prevalence, the exact test of Fisher, the odds ratio and the relative risk. Results: 100 healthy chindren below 10 years old were included (average age 4 years 2 months); 97% stratum 1 and 2; 50% both gender. 30% of the children live in stacking conditions; 84% live in urban zones; 95% have drinking water and 85% have an appropriate disposal of feces. 52% have some kind of animal and 63% attend the kinder garden or school. The prevalence of C. spp in feces was 4% (0.14-7.89% 95% CI). The major prevalence was associated to a non-appropiate feces disposal (OR 0.157 p = 0.106) and to the female gender (p = 0.117). The 25% of the ELISA + tests showed a certain level of malnutrition. Conclusion: The prevalence of the C. spp for the studied population sample was 4%, statistically equal to the prevalence for similar samples of healthy children found in Colombia and other developing countries. The presence of C. spp does not seem to be related to specific symptoms. The association study with social-demographic-type variables suggests that the non appropiate feces disposal is related to higher protozoa prevalence. the anthropometric assessment indicates that malnutrition in children with C. spp is lower (25%) than in children with no C. spp (40.6%).