Although cryopreservation is the standard for autotransplantation, it has logistic and financial disadvantages in undeveloped countries such as Colombia. Between September 1993 and August 2007, in 72 patients, peripheral blood was refrigerated at 4°C up to 144 hours before autotransplantation. 41 men and 31 women of median age 37 years affected with High-risk or relapsing non-Hodgkin lymphoma (NHL) (n = 27, 37.5%), multiple mieloma (MM) (n = 21,29.16%), relapsing Hodgkin disease (n = 12, 16.6%), acute leukemias (n = 8, 11.1%), Langerhans cell histiocytosis (n = 2, 2.8%), breast cancer (n = 1, 1.4%) and autoimmune disease (n = 1, 1.4%) were mobilized with G-CSF only. Conditioning regimens were: MEL 200 in 21 patients, CBV in 28 patients, Cy-TBI in 13 patients, BEAM/BEAC in 7 patients, Fludarabine-MEL-Alemtuzumab in 3 patients. The 17 patients in Group 1 showed pre-refrigeration CFU-GM of 2.62 × 105/kg (range 0.36 to 16.6 × 105/kg) and at re-infusion 1.36 × 105/kg (range 0 to 6.32 × 105/kg) of 83% viability (range, 78% to 96%). These patients showed > 0.5 × 109/L granulocytes on day + 11 (range, 9 to 15) and >20 × 109/L platelets on day +16 (range, 11 to 44). The 49 patients in Group 2 showed CD34 of 5.4 × 106/kg (range, 0.16 to 17.1 × 106/kg) and mononuclear cell count (MNC) of 8.7 × 108/kg, reaching >0.5 × 10 9/L granulocytes at day +13 (range, 9 to 17) and >20 × 109/L platelets on day +15 (range, 10 to 40). Among the 6 patients in Group 3, the average of MNC of 10.73 × 108/kg was reached and >0.5 × 109/L granulocytes on day +11 (range, 10 to 16) and >20 × 109/L platelets on day +14 (range, 10 to 18). No graft failure was observed. No differences were observed between the groups. At three and five years 51% are alive in complete remission. Refrigeration of peripheral blood is a simple, effective, and inexpensive method that can be considered for autotransplants within a few days of harvesting where resources are limited for long-term storage.