Vaginal washings from 118 women were investigated by means of immunofluorescence for the presence of antibodies against Candidaalbicans. A significantly (p < 0.02) higher antibody titer was found in the samples from patients with clinically evident vulvovaginal candidiasis. Vaginal secretions from those patients with a positive culture for C. albicans were assayed by means of Western blotting. A distinct band pattern was found in the group of patients with a positive culture and the classical clinical picture of C. albicans vulvovaginitis.