During a revision of Serpocaulon from Colombia, a new hybrid was found between S. adnatum and S. levigatum near to Manizales city, which is described and illustrated herein. Qualitative and quantitative spore and macro-morphological characters were evaluated using principal component analyses to distinguish the new taxon. Our results suggest that the perispore with leasura, lamina width, rhizome diameter, blade dissection and number of pinnae are important characters to distinguish S. × manizalense. This is the first record of a hybrid between a simple and pinnate-leaved species in Serpocaulon, which is considered to be Critically Endangered (CR).