Abstract This article documents the addition of 283 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Agalinis acuta ; Ambrosia artemisiifolia ; Berula erecta ; Casuarius casuarius ; Cercospora zeae‐maydis ; Chorthippus parallelus ; Conyza canadensis ; Cotesia sesamiae ; Epinephelus acanthistius ; Ficedula hypoleuca ; Grindelia hirsutula ; Guadua angustifolia ; Leucadendron rubrum ; Maritrema novaezealandensis ; Meretrix meretrix ; Nilaparvata lugens ; Oxyeleotris marmoratus ; Phoxinus neogaeus ; Pristomyrmex punctatus ; Pseudobagrus brevicorpus ; Seiridium cardinale ; Stenopsyche marmorata ; Tetranychus evansi and Xerus inauris. These loci were cross‐tested on the following species: Agalinis decemloba; Agalinis tenella; Agalinis obtusifolia; Agalinis setacea; Agalinis skinneriana; Cercospora zeina; Cercospora kikuchii; Cercospora sorghi; Mycosphaerella graminicola; Setosphaeria turcica; Magnaporthe oryzae; Cotesia flavipes; Cotesia marginiventris; Grindelia Xpaludosa; Grindelia chiloensis; Grindelia fastigiata; Grindelia lanceolata; Grindelia squarrosa; Leucadendron coniferum; Leucadendron salicifolium; Leucadendron tinctum; Leucadendron meridianum; Laodelphax striatellus; Sogatella furcifera; Phoxinus eos; Phoxinus rigidus; Phoxinus brevispinosus; Phoxinus bicolor; Tetranychus urticae; Tetranychus turkestani; Tetranychus ludeni; Tetranychus neocaledonicus; Tetranychus amicus; Amphitetranychus viennensis; Eotetranychus rubiphilus; Eotetranychus tiliarium; Oligonychus perseae; Panonychus citri; Bryobia rubrioculus; Schizonobia bundi; Petrobia harti; Xerus princeps; Spermophilus tridecemlineatus and Sciurus carolinensis.