Tuberculosis (TB) and other mycobacteriosis are serious illnesses which control is mainly based on presumptive diagnosis. Besides of clinical suspicion, the diagnosis of mycobacteriosis must be done through genus specific smears of clinical specimens. However, these techniques lack of sensitivity and consequently clinicians must wait culture results as much as two months. Computer analysis of digital images from these smears could improve sensitivity of the test and, moreover, decrease workload of the micobacteriologist. Bacteria segmentation of particular species entails a complex process. Bacteria shape is not enough as a discriminant feature, because there are many species that share the same shape. Therefore the segmentation procedure requires to be improved using the color image information. In this paper we present two segmentation procedures based on fuzzy rules and phase-only correlation techniques respectively that will provide the basis of a future automatic particle' screening.