As part of the management and conservation study at the Chiribiquete National Natural Park developed by the Puerto Rastrojo Foundation, an entomological study was done at the Puerto Abeja Biological Station (Mesay river, Solano municipality, Caqueta department). Jaccard similarity index, Shannon-Vileaver diversity index and attacks rates were calculated for diurnal diptera in each of the six forests evaluated. 31 species of Culicidae, 1 of Triatominae, 1 of Ceratopogonidae, 2 of Simuliidae. 10 of Phlebotomine and 6 of Tabanidae were reported. Three new Culicidae species were reported for Colombia: Sabethes glaucodaemon. Trichoprosopon espini and Uranotaenia pallidoventer and the Caqueta department hematophagous insect's distribution was enlarged. Diurna1 species activity and association between species and forests was obtained. Control measures were recommended aimed at preventing vectorial tropical diseases transmission and the epidemiological importante of potential vectors captured in the area was highlighted.