Objective To evaluate the intra- and inter-rater reliability of the Alberta Infant Motor Scale (AIMS) applied by final level undergraduate physiotherapy students. Materials and methods An assessment of evaluation of diagnostic tests was done in 21 clinically healthy infants of both genders, aged 0 to 18 months, residents at Bucaramanga, Colombia. The evaluation was conducted by 12 raters, 11 physiotherapy students in final level and a physiotherapist with experience in pediatrics. The assessments were performed with an interval of one week and by video. An Intraclass Correlation Coefficient (ICC) was applied for the reliability analysis and Standard Error of Measurement (SEM) was obtained, considering an α=0.05. Results A very good inter-rater reliability for the total score (ICC>0.98) and the sitting subscale (ICC>0.97) were registered. The other subscales showed moderate reliability, ICC between 0.54 and 0.64. Intra-rater reliability showed low to excellent ICC. The SEM was low for the standing, prone subscales and total score (0.20 to 0.77). Conclusions The AIMS is a reliable tool that can be used in our context by undergraduate physical therapy students in their year of study in order to assess motor development in clinically healthy children.