Silver nanoparticles (AgNP) were synthesized by electrochemical method using high purity metallic silver in the presence of the surfactant Pluronic® f127.The synthesized nanoparticles were characterized using UV-Vis spectroscopy, Dynamic Light Scattering (DLS), X-Ray Dispersion Spectroscopy (XDS) and Scanning Electron Microscopy (SEM).The antimicrobial activity of AgNP was evaluated against Escherichia Coli.In presence of surfactant, the prepared nanoparticles led to a monomodal distribution with an average size less than 100 nm.The minimal inhibitory concentration (MIC) of the resulting suspension was evaluated using micro dilution method and the MIC results are satisfactory.