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Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit

Acceso Abierto

ID Minciencias: ART-0000028045-30090

Ranking: ART-ART_A2

Idioma: Inglés

Publicado: 22/10/2014

APC (est): No disponible

Abstract:

To classify 21 new isolates of Trypanosoma cruzi (T. cruzi) according to the Discrete Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to detect the parasite in biological samples. Strains were isolated, DNA extracted, and classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used along with other isolates of various biological samples, for a new PCR using primers designed. Finally, the amplified fragments were sequenced. It was observed the predominance of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no band was obtained when other species were used. This work reveals the genetic variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for detecting the presence of T. cruzi.

Tópico:

Trypanosoma species research and implications

Citaciones:

Citaciones por año:

Altmétricas:

Información de la Fuente:

FuenteAsian Pacific Journal of Tropical Medicine	Cuartil año de publicaciónNo disponible	Volumen7
Issue11	Páginas854 - 859	pISSNNo disponible
ISSN1995-7645	Perfil OpenAlexhttps://openalex.org/S203437272

Enlaces e Identificadores:

Scienti ID	0000028045-30090	Scienti ID	0000159778-1	Minciencias ID	ART-0000028045-30090
Scholar citations URL	https://scholar.google.com/scholar?cites=13230791525830726762&as_sdt=2005&sciodt=0,5&hl=en	Open_access URL	https://doi.org/10.1016/s1995-7645(14)60149-8	Oaipmh URL	https://bibliotecadigital.udea.edu.co/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai:bibliotecadigital.udea.edu.co:10495/35212
Scholar URL	https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=info%3AapRjAss2nbcJ%3Ascholar.google.com&btnG=	Doi URL	https://doi.org/10.1016/s1995-7645(14)60149-8	Pmid URL	https://pubmed.ncbi.nlm.nih.gov/25441983
Dspace URL	https://bibliotecadigital.udea.edu.co/handle/10495/35212	Uri URL	https://hdl.handle.net/10495/35212	Openalex URL	https://openalex.org/W2018505916
Pdf URL	https://www.sciencedirect.com/science/article/pii/S1995764514601498/pdf?md5=5d03721bea5017aef2cce7dcf77cd3fb&pid=1-s2.0-S1995764514601498-main.pdf&_valck=1	Scienti URL	http://www.ncbi.nlm.nih.gov/pubmed/25441983

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