Ten adult cyclic female buffaloes (5 normo-karyotype and 5 hybrids) were treated with a conventional superovulation protocol following 2 luteolytic doses (25 mg, i.m.) of PGF2α 11 days apart (days -11 and 0). In all cases, ovulation post PG treatment was confirmed by ultrasound. Superovulation was initiated on d 12 (~d 10 of cycle) with FSH administered twice daily in decreasing doses/day. Preliminary results obtained here suggest failure in ovulation after the superovulation treatment with FSH in water buffalo during the non breeding season, although the follicular recruitment and further development of ovulatory follicles was evident.