Kaempferol 3-O-[α-L-rhamnopyranosyl (1→6)-/β-D-glucopyranoside] and kaempferol 3-O-[β-D-glucopyranoside] were isolated from the leaves of Hedyosmum bonplandianum H. B. K. (Chloranthaceae), which is used in Colombian folk medicine as an analgesic. The n-butanol extract and the glycosyl flavonoids isolated exhibited significant analgesic activity in mice.