Banana production for export in the Uraba region of Colombia represents economic and social benefits for the country and demands improved agronomic management practices, especially for fertilization. The objective of this research was to evaluate the effect of different doses of nitrogen on crop development during two production cycles in order to adjust fertilizer recommendations to values consistent with plant requirements. Sixth-generation banana Williams plants were subjected to five treatments of nitrogen doses at five stages of development and during two production cycles. Two linear models, according to the phenological stage and vegetative structures, were used, assessing the variables of dry weight and nitrogen content. The statistical differences found for the effect of the nitrogen dose on the dry matter accumulation per plant organ and nitrogen content in the plants for the development stages per production cycle and between the cycles allowed for the selection of the 321.8 and 483 kg ha-1 ha of nitrogen doses as the better ones. In this research, the nitrogen dose of 483 kg ha-1 was the most successful at obtaining the highest nitrogen content in the plants. This research confirmed the practical utility of nutrient extraction curves because they allowed for the selection of the fertilizer dose with the best response.