Background: Ancylostomiasis is a disease produced by the intestinal hematofhagous nematode Ancylostoma caninum. This parasite has as major excretion/secretion protein ASP1, which has a molecular weight of 42KDa and is encoded by a gene of 1016 pb. Methods: RNA of adults of Ancylostoma caninum was amplified by reverse transcription polymerase chain reaction. The ASP1 protein gene was inserted into the pcDNA3 vector. Plasmid was digested with Bamh1 and EcoR1 and cloning was performed directionally. Later on, a transformation and selection of E. coli DH5a cell competent with the product for the ligation was made. Then, a screening by PCR was made to confirm the presence of ASP1 gene. PcDNA3-ASP1 was inoculated by intraglandular parotide and intramuscular route in Balb/c mice. Determination of antibodies in these animals was measured in serum and saliva by ELISA and immunochemistry. Results: PcDNA3-ASP1 was incorporated and expressed in E. coli DH5a cell. This recombinant plásmid induced production of antibodies anti-ASP1 specific in Balb/c mice. Conclusion: It was possible to demonstrate that using of pcDNA3-ASP1 did not display reactogenicity and it did not produce unfavorable reactions, plus it induced a humoral response against the excreción/secretion protein of Ancylostoma caninum in mice. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive