Two methods were evaluated for ADN extraction in plants belonging to the Solanum genus. The objective was to isolate adequate ADN amount and of enough quality for obtaining sequences. The commercial product DNeasy® Plant Mini Kit was compared with a method that includes a lysis buffer. For the last method it was also evaluated the improvement when samples were previously macerated with liquid nitrogen. The quality results (A260/A280) did not show significant differences between the extraction methods (index <1.5). However, there were differences in the ADN concentration obtained (Dunnett test, p <0.05) and in the PCR amplification percentages (X 2 , p <0.05). The best results, in terms of success in PCR (89%) were obtained with the commercial product, however the PCR with 1:100 dilution of extracted ADN using the buffer lysis method achieve comparable results. Grounding the samples with liquid nitrogen with this method, also upgraded the PCR results. Based on the results in this study the lysis buffer method is proposed as a cost-effective alternative for ADN extraction in plants belonging to the evaluated genus.