Bean golden mosaic geminivirus (BGMV) causes major losses on beans (Phaseolus vulgaris) in the tropical and subtropical Americas and the Caribbean Basin. Infectious DNA-A and -B components of BGMV isolates from the Dominican Republic (BGMV-DR) and Guatemala (BGMV-GA) were sequenced, and their genome organizations were similar to those of other whitefly-transmitted geminiviruses from the Western Hemisphere. Whiteflies (Bemisia tabaci) transmitted BGMV from beans infected by particle-gun inoculation with clones of BGMV-DR or -GA. Infectious pseudorecombinants gave symptoms typical of wild-type infections when mixtures of BGMV-GA DNA-A and BGMV-DR DNA-B were used [...]