Fifty-one crude extracts from 31 Colombian plants used in traditional medicine have been screened for cytotoxicity in a MTT assay with V79 cells. Eleven extracts (21%) were active, showing a survival percentage below 20% with respect to the controls. The most active were ethanol and dichloromethane extracts from Acnistus arborescens leaves. The IC50 values obtained (6 and 11 μg/mL) encourage a more in depth investigation.