Summary: N-isopropylacrylamide (NIPAAm)/butylacrylate (BAc) copolymer was synthesized by emulsion polymerization in order to use it as a cell culture surface for corneal epithelium biosubstitutes. Results showed that the obtained polymers were thermosensitive hydrogels. The copolymer was characterized by Fourier transform infrared (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and swelling degree. 3T3 Swiss cells were used as feeder layers and they were used to test the cytotoxic effect of the hydrogels. The conditions to isolate, cultivate, expand and cryo-preserve human oral mucosal cells were established and analysis of several morphological and immuno-histochemical characteristics of the cultivated oral mucous were made.