The article indicates the potential of some motion pictures as mediators in the denaturalization of sexuality and gender issues. It also offers a reflection on the pertinence and contribution of this matter for the teachers’ education. Based in the Cultural Studies, this analysis highlights the importance of cinema for building the individual cultural identity. The examination of sequences of three cinematographic productions from the Sixties and Seventies — Chuvas de Verão, by Cacá Diegues; Julieta dos Espíritos, by Federico Fellini; and Vivre sa Vie, by Jean Luc Godard — supports the deconstruction of stereotypes and redeem the complexity of the sexuality and gender issues. As object of reflection and analysis by teachers, cinema shows to be a tool capable of leveraging change and meaning shifts of life and social roles.