Abstract Background Trypanosoma cruzi, the etiological agent of Chagas’ disease , is an obligate intracellular parasite which induces a CD8 + T cell immune response with secretion of cytokines and release of cytotoxic granules. Although an immune-suppressive effect of T. cruzi on the acute phase of the disease has been described, little is known about the capacity of CD8 + T cell from chronic chagasic patients to respond to a non- T. cruzi microbial antigen. Methods In the present paper, the frequency, phenotype and the functional activity of the CD8 + T cells specific from Flu-MP*, an influenza virus epitope, were determined in 13 chagasic patients and 5 healthy donors. Results The results show that Flu-MP* peptide specific CD8 + T cells were found with similar frequencies in both groups. In addition, Flu-MP* specific CD8 + T cells were distributed in the early or intermediate/late differentiation stages without showing enrichment of a specific sub-population. The mentioned Flu-MP* specific CD8 + T cells from chagasic patients were predominately T EM (CCR7- CD62L-), producing IL-2, IFNγ, CD107a/b and perforin, and did not present significant differences when compared with those from healthy donors. Conclusions Our results support the hypothesis that there is no CD8 + T cell nonspecific immune-suppression during chronic Chagas disease infection. Nonetheless, other viral antigens must be studied in order to confirm our findings.