Inversion of high-frequency acoustic reflection data in low-noise layered sea bottoms is presented. The assumed geoacoustic model includes attenuation effects. An overall attenuation coefficient of the whole sedimentary column is estimated by logarithmic regression on the spectral ratio of nonoverlapped replicas. The attenuation response is filtered out of the impulse response by the minimum square inversion of the sea bottom lossy transfer function, previously computed by deconvolving the input pulse from the reflection response. Applying this inversion scheme to a known artificial bottom, experimental values lying within a 7.4% range below the actual data are obtained.