A study was done where the viability of the M. tuberculosis in sputum was not affected by the addition of ten per cent of trisodium phosphate (TSP) at 10% in equal parts, when it is used as a decontaminating agent, maintained at room temperature for 48 hours. Nor was its viability altered when it was grown in ogawa/Kudoh (O-K) medium, after having been kept for up to a month at room temperature (20: 2°C).