Background: Subtyping of Human Papilloma Virus (HPV) by molecular biology tools may enhanced cervical cytological assessments information for patient́s management and for prognosis of cervical cancer evolution. The aim of this study was to subtype HPV from cervical samples of sexually active women (15-69yr-old) seeing in cervic-gyn public clinic (IHAULA) of Merida State, Venezuela. Methods: DNA-collection device (Digene®) were used to collect and transport 250 cervical smears, DNA were isolated using QIAamp® DNA Mini Kit (Qiagen®) and storage at −20 °C until used. A Nested-PCR-Multiplex assay was standardized for amplification of early E6/E7 HPV virus. A first run included amplification of a 630bp-length region of E6/E7 gene and a second run allowed to genotype HPV virus in a multiplex format. Amplicons sizes varied from 151-457 bp for HPV-16,-18,-31,-45; and HPV-33,-6/11,-58,-52,-56. Simultaneously, HPV detection assays were performed using Hybrid-Capture II (Digene®) technology and an endpoint PCR assays for L1 and E6/E7 regions. Results: HPV were detected in 27.2% samples, 94.12% (64/68) were positive for at least one of the genotypes assayed. High risk HPV were identify in 98.44% (63/64) samples; where HPV18 (50/63) was the most common genotype isolated, along with HPV16 (24/63). One or several types were detected in 56.25% (36/64) of the cases, being VPH-18-6/11 (14/64) combination the most common one. Conclusion: we found a high frecuency of cervical infection with high-oncogenic-risk HPV genotypes, specially caused by HPV18, alone or in multiple types infection. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive